Send Message

Provide Nanoparticle Reagents and Conjugation Technology 

Sales & Support
Request A Quote - Email
Select Language
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Home News

PEG Liners- PFP (PEG)n Reagents

I'm Online Chat Now
China Wuhan Borenpharm Co., Ltd. certification
China Wuhan Borenpharm Co., Ltd. certification
We tested the 2,4,5-trimethyl-2,5-dihydro-1,3-thiazole in our assay and the results look good. It produced the expected stress response in our mice.

—— Kevin

Company News
PEG Liners- PFP (PEG)n Reagents


The Borenpharm PFP (PEG)n are PEG labeling reagents that react with primary and secondary amines. The pentafluorophenyl (PFP) ester-activated PEG linker is less subject to hydrolysis than NHS esters, resulting in more efficient reactions. PFP (PEG)n must be first dissolved in a minimal amount of an organic solvent, such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF) and then added to the buffer containing the protein or other molecule. The reagent forms an emulsion that allows the reaction to proceed.

Important Product Information

PFP (PEG)n is moisture-sensitive. Store the vial of reagent at 4°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.

As directed in the procedure, dissolve the PEG reagent immediately before use. The PFP moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time, and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction. If necessary, dialyze or desalt to exchange the protein sample into an amine-free buffer such as phosphate-buffered saline (PBS).

When Azidolating proteins in solution, excess nonreacted linker is easily removed by size exclusion using either desalting columns or dialysis. A 10mL desalting column is best suited for processing PEGlation reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller amounts of protein and/or smaller reaction volumes, both the azidolation reaction and subsequent buffer exchange may be performed in a single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For larger reaction volumes than can be processed with a desalting column, either split the sample between two columns or use an appropriate Slide-A-Lyzer® Dialysis Cassette.

Pub Time : 2017-11-07 15:18:48 >> News list
Contact Details
Wuhan Borenpharm Co., Ltd.

Contact Person: cathy

Tel: 13240061617

Fax: 86-27-59218822

Send your inquiry directly to us