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CAS#: | 564476-33-1 | M.F.: | C15H30O6S |
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M.W.: | 338.46 | Appearance: | Transparent And Oil Free Liquid |
Purity: | 95% Min | Storage: | -20℃ For Long Term, Keep Cool And Dry |
Highlight: | peg chemical,peg polyethylene |
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 Of PEG Linker Is Applicated In Medical Research
Item#:. | BK02868 |
CAS#: | 564476-33-1 |
M.F.: | C15H30O6S |
M.W.: | 338.46 |
Pruity: | >95% |
Storage: | -20℃ for Long Term |
Appearance: | Liquid |
Introduction:
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 is a reagent with an extended spacer arm. This reagent is soluble in organic solvents such as DMSO or DMF. Once dissolved in an organic solvent, the reagent is further diluted in a non-amine containing aqueous buffer. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Because antibodies and other proteins generally contain multiple lysine (K) residues in addition to the N-terminus of each polypeptide, they have multiple primary amines available as targets for labeling with NHS-activated PEG reagents.
Important Product Information:
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 is moisture-sensitive. Store the vial of the reagent at -20°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.
Dissolve NHS-PEG-linker immediately before use. The NHS-ester moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
• Avoid buffers containing primary amines (e.g., Tris or glycine) as these compete with the intended reaction. If necessary, dialyze or otherwise desalt to exchange the protein sample into an amine-free buffer such as phosphate buffered saline.
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