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|M.W.:||338.46||Appearance:||Transparent And Oil Free Liquid|
|Purity:||95% Min||Storage:||-20℃ For Long Term, Keep Cool And Dry|
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 Of PEG Linker Is Applicated In Medical Research
|Storage:||-20℃ for Long Term|
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 is a reagent with an extended spacer arm. This reagent is soluble in organic solvents such as DMSO or DMF. Once dissolved in an organic solvent, the reagent is further diluted in a non-amine containing aqueous buffer. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Because antibodies and other proteins generally contain multiple lysine (K) residues in addition to the N-terminus of each polypeptide, they have multiple primary amines available as targets for labeling with NHS-activated PEG reagents.
Important Product Information:
Thiol-PEG4-t-butyl ester With CAS NO.564476-33-1 is moisture-sensitive. Store the vial of the reagent at -20°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.
Dissolve NHS-PEG-linker immediately before use. The NHS-ester moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
• Avoid buffers containing primary amines (e.g., Tris or glycine) as these compete with the intended reaction. If necessary, dialyze or otherwise desalt to exchange the protein sample into an amine-free buffer such as phosphate buffered saline.
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