CAS 1263166-93-3 BCN POE3 NH2 C17H28N2O4 Low Melting Solids

Protein Pegylation Protocol / Peg Lipid BCN - POE3- NH2 With CAS 1263166-93-3

Application: used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. Modification of proteins adds polyethylene glycol spacers, which impart increased water solubility, reduced immunogenicity of the labeled molecule and enhanced in vivo stability in solution. Functionalization of solid surfaces, such as quantum dots, self-assembled monolayers and nanoparticles, with polyethylene glycol spacers significantly reduces nonspecific protein binding. (PEG)5 amine reagents used with (PEG)5 amine reagents in surface modification can form a hydrophilic “lawn” of methyl ether-terminated PEGs with periodic exposed carboxy-terminated PEGs. The exposed carboxy groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Typical PEGylation reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.

Important Product Information

BCN-POE3-NH2 With CAS.1263166-93-3 is low-melting solids.

• Use the BCN-POE3-NH2 in combination with (PEG)n amine, reagents to modify surfaces and minimize nonspecific binding.

• Use non-amine-containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 20mM HEPES; 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.