Amino-PEG3-T-Butyl ester With Cas.252881-74-6 Is For Chemical Modification

Amino-PEG3-T-Butyl ester With Cas.252881-74-6 Is For Chemical Modification

Synonyms:

TERT - BUTYL 12 - AMINO - 4 7 10 - TRIOXA - DODECA ; H2N - PEG3 - tBu ; H2N - PEG3 -CH2CH2COOtBu ; tert - Butyl 12 - aMino - 4 , 7 , 10 - trioxa - dodec ; tert - butyl 3 - { 2 - [ 2 - ( 2 -hydroxyethoxy ) ethoxy ] ethoxy } propanoate ; 3 - [ 2 - [ 2 - ( 2 - Aminoethoxy ) ethoxy ] ethoxy ] propionic acid tert-butyl ester ; Propanoic acid , 3 - [ 2 - [ 2 - ( 2 - aMinoethoxy ) ethoxy ] ethoxy ] -, 1, 1 - diMethylethyl ester ; tert - Butyl 12 - amino - 4 , 7 , 10 - trioxadodecanoate.

Introduction

The Amino-PEG3-T-Butyl ester With Cas.252881-74-6 is used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. Modification of proteins adds polyethylene glycol (PEG)_n spacers, which impart increased water solubility, reduced immunogenicity of the labeled molecule and enhanced in vivo stability in solution. Functionalization of solid surfaces, such as quantum dots, self-assembled monolayers and nanoparticles, with polyethylene glycol spacers significantly reduces nonspecific protein binding. (PEG)n amine reagents used with (PEG)n amine reagents in surface modification can form a hydrophilic “lawn” of methyl ether-terminated PEGs with periodic exposed carboxy-terminated PEGs. The exposed carboxy groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Typical PEGylation reagents contain heterogeneous mixtures of different PEG chain lengths; however, our PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimizing modification applications.

Important Product Information

• The Amino-PEG3-T-Butyl ester With Cas.252881-74-6 is low-melting solids that are difficult to weigh and dispense. To facilitate handling, make a stock solution by dissolving the reagent with dimethylsulfoxide (DMSO) or dimethylformamide (DMF).

• Use the (PEG)n amine reagents in combination with (PEG)n amine reagents to modify surfaces and minimize nonspecific binding.

• Use non-amine-containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 20mM HEPES; 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.