Thiol-PEG3-Propargyl Of Alkyne PEG Is Widely Applied In PEGylation

Thiol-PEG3-Propargyl​ Of Alkyne PEG Is Widely Applied In PEGylation

Description:

Thiol-PEG3-Propargyl can be used to modify proteins, peptides and other materials via amino or other acid reactive chemical groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

       Because free thiol (SH) groups, also called mercapto groups, are not present as abundantly as amino groups in most biopolymers such as proteins and nucleic acids, thiol-reactive reagents often provide a means of selectively modifying a protein at a defined site. Therefore thiol-reactive dyes are often used to prepare fluorescent peptides, proteins and oligonucleotides for probing biological structures, functions and interactions. Thiol-reactive dyes have been used to develop probes for analyzing the topography of proteins in biological membranes, determining distances within the protein or between the proteins and monitoring the changes in protein conformation using environment-sensitive probes.

      There are many types of thiol-reactive dyes reported in the literature, including iodoacetamides, disulfides, maleimides, vinyl sulfones and various electron-deficient aryl halides and sulfonates. Iodoacetamides and maleimides are by far the most popular thiol-reactive moieties. Iodoacetamides and maleimides readily react with thiol moieties of biopolymers to form thioether conjugates. The thioether bond formed is quite stable. Additionally, iodoacetamides and maleimides have good selectivity to thiol groups. However, they may also react with histidine or potentially tyrosine under higher pH if free thiols are not readily available. Iodo compounds are known to be very light-sensitive, especially in solution. Thus, we recommend the reactions of iodoacetamides with biomolecules should be carried out under subdued light. The bioconjugation reactions of thiol-reactive probes can be quenched by the addition of cysteine, glutathione, or mercaptosuccinic acid to the reaction mixture, forming highly water-soluble adducts that are easily removed by dialysis or gel filtration .

Physical Properties:

• Transparent and oil free liquid;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, dessiccated Protect from light. Avoid frequent thaw and freeze.