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Product Details:
Payment & Shipping Terms:
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M.F.: | C11H16BrNO6 | M.W.: | 338.15 |
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Appearance: | Transparent And Oil Free Liquid | Purity: | 95% Min |
Storage: | -20℃ For Long Term, Keep Cool And Dry | Item#: | BK02107 |
High Light: | nhs ester,nhs chemical |
Bromo-PEG2-NHS ester Of NHS ester PEG Is For Targeted Drug Delivery
Item#:
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BK02107 |
CAS#:
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M.F.:
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C11H16BrNO6 |
M.W.:
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338.15 |
Appearance:
|
Transparent and oil free liquid |
Purity:
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95% |
Storage:
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-20℃ for Long Term, Keep cool and dry |
Important Product Information
• Bromo-PEG2-NHS ester is moisture-sensitive. Store the vial of biotin reagent at 4°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.
• As directed in the procedure, dissolve the NHS ester reagent immediately before use. The NHS moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time, and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
• Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction. If necessary, dialyze or desalt to exchange the protein sample into an amine-free buffer such as phosphate-buffered saline (PBS).
• When Pegylating proteins in solution, excess non-reacted PEG linker is easily removed by size exclusion using either desalting columns or dialysis (Additional Information). A 10mL desalting column is best suited for processing Pegylation reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller amounts of protein and/or smaller reaction volumes, both the Pegylation reaction and subsequent buffer exchange may be performed in a single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For larger reaction volumes than can be processed with a desalting column, either split the sample between two columns.
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