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Borenpharm C31H39ClN2S Cy5 Peg SH Bis NHS PEG 95% min Purity

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China Wuhan Borenpharm Co., Ltd. certification
China Wuhan Borenpharm Co., Ltd. certification
We tested the 2,4,5-trimethyl-2,5-dihydro-1,3-thiazole in our assay and the results look good. It produced the expected stress response in our mice.

—— Kevin

Borenpharm C31H39ClN2S Cy5 Peg SH Bis NHS PEG 95% min Purity

Borenpharm C31H39ClN2S Cy5 Peg SH Bis NHS PEG 95% min Purity
Borenpharm C31H39ClN2S Cy5 Peg SH Bis NHS PEG 95% min Purity

Large Image :  Borenpharm C31H39ClN2S Cy5 Peg SH Bis NHS PEG 95% min Purity Get Best Price

Product Details:

Place of Origin: China
Brand Name: Borenpharm
Certification: www.borenpharm.com
Model Number: BK02175

Payment & Shipping Terms:

Minimum Order Quantity: 1g
Price: negotiable
Packaging Details: 1g 5g 10g 25g 50g
Delivery Time: Within 24 hours
Supply Ability: in stock
Detailed Product Description
M.F.: C31H39ClN2S M.W.: 507.17
Appearance: Transparent And Oil Free Liquid Purity: 95% Min
Storage: -20℃ For Long Term, Keep Cool And Dry Item#: BK02175
High Light:

C31H39ClN2S Cy5 Peg SH

,

Borenpharm Cy5 Peg SH

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Bis NHS PEG 95%

Cy5-SH Of NHS ester PEG Is For Targeted Drug Delivery​

Item#:
BK02175
CAS#:
 
M.F.:
C31H39ClN2S
M.W.:
507.17
Appearance:
Transparent and oil free liquid
Purity:
95%
Storage:
-20℃ for Long Term, Keep cool and dry

 

The entire conjugation can be performed in about a half-day. In addition to the materials listed below, you will need to have a solution of your antibody at a concentration (optimally) of at least 2 mg/ml. The extent of Cy5 conjugation to the antibody may depend on the concentration of antibody in solution; for consistent conjugations, use a consistent concentration. You should be familiar with how to use a desalting column and how to take absorbance spectra.

The reactive Cy5 molecule is unstable. Open a vial, and weigh out the amount you need (typically, 1 or 2 mg is more than enough). Reseal the vial and store under dessicant at 4C. Immediately disoolve the Cy5 in DMSO at a concentration of 10 mg/ml.

When first conjugating an antibody, a range of Cy5 to antibody concentrations should be compared. We recommend molar ratios of 3, 5, and 7 to start with. Compare each conjugate by staining (you should perform a titration of antibody on cells for each reagent to determine the optimal staining concentration). Select the conjugate with the brightest "positive" cells which still has low background on "negative" cells.

 

Important Product Information

• Cy5-SH is moisture-sensitive. Store the vial of biotin reagent at 4°C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.

• As directed in the procedure, dissolve the NHS ester reagent immediately before use. The NHS moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time, and do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

• Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction. If necessary, dialyze or desalt to exchange the protein sample into an amine-free buffer such as phosphate-buffered saline ( PBS ) .

• When Pegylating proteins in solution, excess non-reacted PEG linker is easily removed by size exclusion using either desalting columns or dialysis (Additional Information). A 10mL desalting column is best suited for processing Pegylation reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller amounts of protein and/or smaller reaction volumes, both the Pegylation reaction and subsequent buffer exchange may be performed in a single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For larger reaction volumes than can be processed with a desalting column, either split the sample between two columns.

Contact Details
Wuhan Borenpharm Co., Ltd.

Contact Person: cathy

Tel: 13240061617

Fax: 86-27-59218822

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